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Describe five different essential components of a PCR reaction and explain the purpose of each one. f (5 marks) (d) If you start with one double-stranded DNA template in a PCR reaction, how many copies of the exact double-stranded PCR product could you expect to have by the start of the fourth denaturation step? Explain your reasoning with the help of a diagram. (4 marks) (e) Design a pair of mutagenesis primers to mutate Val12 in Protein Sequence B to alanine. Explain your answer. (5 marks) (a) DNA sequence A encodes Protein sequence B which is a DNAbinding sub-unit of human RNA polymerase II. Explain the specific purpose of this enzyme and why it must bind to DNA. (3 marks) (b) Design forward and reverse primers that could be used to amplify the entire DNA sequence A and clone it into an expression vector. Include a BamHI restriction site (GGATCC) on the forward primer and an Ndel restriction site (CATATG) on the reverse primer. Decide whether you need to include a stop codon. Explain your answer. (8 marks) (c) Describe five different essential components of a PCR reaction and explain the purpose of each one. f (5 marks) (d) If you start with one double-stranded DNA template in a PCR reaction, how many copies of the exact double-stranded PCR product could you expect to have by the start of the fourth denaturation step? Explain your reasoning with the help of a diagram. (4 marks) (e) Design a pair of mutagenesis primers to mutate Val12 in Protein Sequence B to alanine. Explain your answer. (5 marks) Specific Purpose of Enzyme Essay PaperThis question has already been tackled by one of our writers and a good grade recorded. You can equally get high grades by simply making your order for this or any other school assignment that you may have.
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